59 research outputs found
Application of Artificial Neural Networks in Predicting Abrasion Resistance of Solution Polymerized Styrene-Butadiene Rubber Based Composites
Abrasion resistance of solution polymerized styrene-butadiene rubber (SSBR)
based composites is a typical and crucial property in practical applications.
Previous studies show that the abrasion resistance can be calculated by the
multiple linear regression model. In our study, considering this relationship
can also be described into the non-linear conditions, a Multilayer Feed-forward
Neural Networks model with 3 nodes (MLFN-3) was successfully established to
describe the relationship between the abrasion resistance and other properties,
using 23 groups of data, with the RMS error 0.07. Our studies have proved that
Artificial Neural Networks (ANN) model can be used to predict the SSBR-based
composites, which is an accurate and robust process
User-friendly optimization approach of fed-batch fermentation conditions for the production of iturin A using artificial neural networks and support vector machine
Background: In the field of microbial fermentation technology, how to
optimize the fermentation conditions is of great crucial for practical
applications. Here, we use artificial neural networks (ANNs) and
support vector machine (SVM) to offer a series of effective
optimization methods for the production of iturin A. The concentration
levels of asparagine (Asn), glutamic acid (Glu) and proline (Pro)
(mg/L) were set as independent variables, while the iturin A titer
(U/mL) was set as dependent variable. General regression neural network
(GRNN), multilayer feed-forward neural networks (MLFNs) and the SVM
were developed. Comparisons were made among different ANNs and the SVM.
Results: The GRNN has the lowest RMS error (457.88) and the shortest
training time (1 s), with a steady fluctuation during repeated
experiments, whereas the MLFNs have comparatively higher RMS errors and
longer training times, which have a significant fluctuation with the
change of nodes. In terms of the SVM, it also has a relatively low RMS
error (466.13), with a short training time (1 s). Conclusion: According
to the modeling results, the GRNN is considered as the most suitable
ANN model for the design of the fed-batch fermentation conditions for
the production of iturin A because of its high robustness and
precision, and the SVM is also considered as a very suitable
alternative model. Under the tolerance of 30%, the prediction
accuracies of the GRNN and SVM are both 100% respectively in repeated
experiments
A gene-based recessive diplotype exome scan discovers \u3cem\u3eFGF6\u3c/em\u3e, a novel hepcidin-regulating iron-metabolism gene
Standard analyses applied to genome-wide association data are well designed to detect additive effects of moderate strength. However, the power for standard genome-wide association study (GWAS) analyses to identify effects from recessive diplotypes is not typically high. We proposed and conducted a gene-based compound heterozygosity test to reveal additional genes underlying complex diseases. With this approach applied to iron overload, a strong association signal was identified between the fibroblast growth factor–encoding gene, FGF6, and hemochromatosis in the central Wisconsin population. Functional validation showed that fibroblast growth factor 6 protein (FGF-6) regulates iron homeostasis and induces transcriptional regulation of hepcidin. Moreover, specific identified FGF6variants differentially impact iron metabolism. In addition, FGF6 downregulation correlated with iron-metabolism dysfunction in systemic sclerosis and cancer cells. Using the recessive diplotype approach revealed a novel susceptibility hemochromatosis gene and has extended our understanding of the mechanisms involved in iron metabolism
Iron Metabolism Regulates p53 Signaling through Direct Heme-p53 Interaction and Modulation of p53 Localization, Stability, and Function
Iron excess is closely associated with tumorigenesis in multiple types of human cancers, with underlying mechanisms yet unclear. Recently, iron deprivation has emerged as a major strategy for chemotherapy, but it exerts tumor suppression only on select human malignancies. Here, we report that the tumor suppressor protein p53 is downregulated during iron excess. Strikingly, the iron polyporphyrin heme binds to p53 protein, interferes with p53-DNA interactions, and triggers both nuclear export and cytosolic degradation of p53. Moreover, in a tumorigenicity assay, iron deprivation suppressed wild-type p53-dependent tumor growth, suggesting that upregulation of wild-type p53 signaling underlies the selective efficacy of iron deprivation. Our findings thus identify a direct link between iron/heme homeostasis and the regulation of p53 signaling, which not only provides mechanistic insights into iron-excess-associated tumorigenesis but may also help predict and improve outcomes in iron-deprivation-based chemotherapy
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